mouse monoclonal antibody ox 42  (Bio-Rad)

Journal: Frontiers in Aging Neuroscience

Article Title: Spinal glial cell derived extra-pituitary prolactin contributes to postoperative pain in females

doi: 10.3389/fnagi.2026.1741102

Figure Lengend Snippet: PRL expression in the spinal cord of sham- and incision-operated female rats and mice. (A, B) PRL/OX-42 (A) and PRL/NeuN co-labeling in the ipsilateral spinal cord (SC) of female rats 1 day after sham. Cyan arrows mark PRL + astrocyte-like cells. NeuN was used as a pan-neuronal marker. Scale bars: 50 μm. (C, C′) PRL labeling in SC 1 day after sham and incision (INC) procedure. Images show dorsal horn regions (laminae I–V; partial ventral laminae). Images were captured with a 10 × objective (A, B, C, C′) . (D) PRL + density in the ipsilateral dorsal horn of SC 1 day after sham and incision (INC) procedure. (E, E′) Immunolabeling of PRL in laminae I–II of the ipsilateral spinal cord (SC) from female mice 1 day after sham surgery (Sham). Yellow arrow indicates OX42 + microglia, and pink arrows indicate PRL − /GFAP + astrocytes. (F, F′) PRL labeling in laminae I–II of the ipsilateral SC from female mice 1 day after incision (INC). Yellow arrow indicates OX42 + microglia, and cyan arrows indicate PRL + /GFAP + astrocytes. Surgical procedures are indicated in row titles. Antibodies and corresponding fluorescence colors are shown in the photomicrographs. Images were captured with a 40 × objective. Scale bar: 25 μm for all panels (E-F′) . (G) Percentages of PRL + cells in NeuN + , Ox-42 + and GFAP + in female mice 1 days after INC.

Article Snippet: The following antibodies were used in the study: PRL protein in rats was detected with rabbit anti-PRL (Agilent-DAKO, Santa Clara, CA; cat: A0569; 1:100) ( ); PRL in mice was detected with rabbit anti-PRL (Bioss, Boston, MA; cat: BS-23763R; 1:200); pSTAT5 in rats was detected with rabbit anti-pSTAT5 (Tyr694, Cell Signaling Technology, Beverly, MA; cat: 9314S; 1:200) ( ); rat DRG and spinal cord neurons were detected with mouse monoclonal anti-NeuN antibodies (Millipore-Sigma, St. Louis, MO; catalog MAB377; 1:100); mouse spinal astrocytes and Schwann cells in hind paw were labeled with chicken anti-GFAP (Neuromics, Edina, MN; catalog CH22102; 1:100); and rat and mouse monocytes/macrophages/dendritic cells/microglia were labeled with mouse monoclonal antibody OX-42 (CD11b/c; Bio-Rad, Hercules, CA; catalog MCA275GA; 1:50) ( ).

Techniques: Expressing, Labeling, Marker, Immunolabeling, Fluorescence

Journal: BMC Pharmacology

Article Title: Effect of a short-term in vitro exposure to the marine toxin domoic acid on viability, tumor necrosis factor-alpha, matrix metalloproteinase-9 and superoxide anion release by rat neonatal microglia

doi: 10.1186/1471-2210-1-7

Figure Lengend Snippet: Immunofluorescent visualization of CD11b/c and ionotropic glutamate receptor subunit GluR4 in neonatal rat brain microglia. The integrin CD11b/c and the ionotropic glutamate receptor subunit GluR4 were visualized using the dual-labeling indirect immunofluorescent technique (See Materials and Methods). (Top left) bright field, phase contrast view of microglia incubated in the absence of the primary antibodies against GluR4 and CD11b/c. (Top right) same microglia viewed using FITC filter configuration. Note vesicular structures exhibiting intense autofluorescence, but little or no fluorescence over most of the microglia cell bodies or the elongated processes. (Middle left) bright field, phase contrast view of microglia labeled with anti-glutamate receptor GluR4 subunit and the leukocyte marker CD11b/c. (Middle right)(green) same field viewed using the FITC filter configuration. Microglia not only contain fluorescent vesicles, but exhibit a prominent speckled pattern of fluorescence over the cell bodies and the elongated processes. Absence of speckled pattern of labeling in cells incubated without primary GluR4 antibody (compare with top right photo) suggests the speckled pattern represents specific labeling of GluR4 subunit. (Lower left) same microglia exhibited intense labeling of CD11b/c (red), which confirms microglia are of leukocytic origin. (Bottom right ) GluR4 and CD11b/c superimposed labeling (yellow). Approximate magnification of the original was × 525.

Article Snippet: Hank's balanced salt solution (HBSS), penicillin (P), streptomycin (S), trypsin (0.25%)-EDTA (1 mM) and trypan blue were purchased from GIBCO-BRL (Grand Island, NY); certified heat-inactivated fetal bovine serum (FBS) was obtained from Hyclone (Logan, UT); mouse anti-rat CD11b/c monoclonal antibody (OX-42) and rabbit anti-glutamate receptor (GluR4) polyclonal antibody were purchased from Pharmingen (San Diego, CA).

Techniques: Labeling, Incubation, Fluorescence, Marker